Cap and insert the exhaust hose of the cover into the exhaust groove of the inner sterilization tank. Then tighten the two bolts at the same time in a symmetrical manner to make the bolts tight and not leaking.
Heat with an electric stove or gas and open the exhaust valve at the same time to boil the water to remove the cold air from the pot. After the cold air is completely drained, close the exhaust valve so that the temperature inside the pot gradually increases as the steam pressure increases. When the pressure in the pot rises to the desired pressure, the heat source is controlled to maintain the pressure for the required time. This experiment was sterilized using 1.05 kg/cm2 at 121.3 °C for 20 minutes.
After the time required for sterilization, turn off the power or turn off the gas, let the temperature inside the sterilizer drop naturally. When the pressure of the pressure gauge drops to 0, open the vent valve, loosen the bolt, open the lid, and take out the sterilized items. If the pressure does not drop to 0, open the exhaust valve, the pressure in the pot will drop suddenly, so that the medium in the container will be flushed out of the flask mouth or the test tube mouth due to the imbalance of internal and external pressure, causing the cotton plug to contaminate the medium. Pollution.
The sterilized medium taken out was placed in a 37 ° C incubator for 24 hours, and it was checked if no bacteria were grown.